US 12,220,675 B2
Device and method for mixing and bubble removal
David R. Almassian, Gaithersburg, MD (US); William M. Nelson, Rockville, MD (US); and Aymeric Randanne de Vazeille, Leesburg, VA (US)
Assigned to Tetracore, Inc., Rockvile (MD)
Filed by Tetracore, Inc., Rockville, MD (US)
Filed on Feb. 11, 2021, as Appl. No. 17/173,315.
Application 17/173,315 is a division of application No. 15/255,891, filed on Sep. 2, 2016, granted, now 10,953,376.
Claims priority of provisional application 62/213,669, filed on Sep. 3, 2015.
Prior Publication US 2021/0162357 A1, Jun. 3, 2021
Int. Cl. B01F 33/452 (2022.01); B01D 19/00 (2006.01); B01F 33/45 (2022.01); B01F 33/451 (2022.01); B01F 35/22 (2022.01); B01F 35/32 (2022.01)
CPC B01F 33/452 (2022.01) [B01D 19/0052 (2013.01); B01F 33/45 (2022.01); B01F 33/451 (2022.01); B01F 35/2207 (2022.01); B01F 35/3202 (2022.01); B01F 35/3204 (2022.01)] 11 Claims
OG exemplary drawing
 
1. A kit for amplifying DNA from dried reaction components, the kit comprising (a) a magnetic mixing device, (b) dried down reaction components, and (c) a thermocycler for DNA amplification, wherein the magnetic mixing device comprises:
a reaction chamber, for mixing the reaction components;
a magnetic stir bar within the reaction chamber;
a holder containing a magnet embedded in an end face of the holder, the magnet being magnetically couplable to the magnetic stir bar; and
a source of motive power coupled to the holder to rotate the holder about a rotation axis, the holder positioned adjacent to the reaction chamber, and the rotation axis being perpendicular to a longitudinal axis of the reaction chamber and extending below a bottom of the reaction chamber,
wherein rotation of the holder causes movement of the magnetic stir bar in a direction vertical with respect to the bottom of the mixing device and along the longitudinal axis of the least one reaction chamber.