US 12,220,452 B2
Cellular adjuvants for viral infection
Kayvan Niazi, Culver City, CA (US); Raymond Wong, Los Angeles, CA (US); Peter Sieling, Culver City, CA (US); and Philip T. Liu, Culver City, CA (US)
Assigned to NantBio, Inc., Culver City, CA (US)
Filed by NantBio, Inc., Culver City, CA (US)
Filed on Mar. 1, 2022, as Appl. No. 17/684,121.
Application 17/684,121 is a continuation of application No. 16/536,962, filed on Aug. 9, 2019, granted, now 11,298,414.
Claims priority of provisional application 62/717,560, filed on Aug. 10, 2018.
Prior Publication US 2022/0370587 A1, Nov. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/02 (2006.01); A61K 39/00 (2006.01); A61K 39/108 (2006.01); C12N 15/861 (2006.01); A61K 9/00 (2006.01)
CPC A61K 39/0258 (2013.01) [A61K 39/0002 (2013.01); C12N 15/861 (2013.01); A61K 9/0019 (2013.01); A61K 2039/605 (2013.01); C12N 2770/16034 (2013.01)] 19 Claims
 
1. A method of increasing expression of a recombinant antigen in a dendritic host cell, comprising:
providing an adenovirus having a genome that includes a recombinant sequence encoding an antigen, wherein the sequence is operably linked to a promoter to drive expression of the antigen in the dendritic host cell;
providing a non-host cell, wherein the non-host cell is a Bacille Calmette Guerin, attenuated (BCG); and
ex vivo transfecting the recombinant adenovirus into the dendritic host cell;
wherein the step of ex vivo transfecting comprises concurrently exposing the dendritic host cell with both the adenovirus and the non-host cell under conditions to allow uptake of the virus into the host cell and to allow for expression of the recombinant sequence in the host cell;
wherein the exposure of the dendritic host cell with the non-host cell is in an amount that increases expression of the antigen in the dendritic cell as compared to exposure of the dendritic host cell with the adenovirus alone; and
wherein the dendritic host cell is transfected with BCG at an MOI (multiplicity of infection) of less than 5.0.