US 12,220,430 B2
Method for killing colorectal cancer cells
Suriya Rehman, Dammam (SA); and Rabindran Jermy, Dammam (SA)
Assigned to Imam Abdulrahman Bin Faisal University, Dammam (SA)
Filed by Imam Abdulrahman Bin Faisal University, Dammam (SA)
Filed on Apr. 17, 2023, as Appl. No. 18/301,505.
Application 18/301,505 is a division of application No. 17/231,231, filed on Apr. 15, 2021, abandoned.
Prior Publication US 2023/0248763 A1, Aug. 10, 2023
Int. Cl. A61K 36/06 (2006.01); A61K 9/51 (2006.01); A61K 33/08 (2006.01); A61K 33/24 (2019.01); A61K 33/38 (2006.01); A61K 36/07 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01)
CPC A61K 33/24 (2013.01) [A61K 9/51 (2013.01); A61K 9/5192 (2013.01); A61K 33/08 (2013.01); A61K 33/38 (2013.01); A61K 36/06 (2013.01); A61K 36/07 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01)] 5 Claims
 
1. A method of making TiO2 nanoparticles, comprising:
drying a Fomitopsis pinicola sample to form a F. pinicola powder,
mixing the F. pinicola powder with water to form a F. pinicola precursor,
sonicating the F. pinicola precursor then centrifuging to obtain a F. pinicola extract;
reacting the F. pinicola extract with titanium isopropoxide to form the TiO2 nanoparticles; and
isolating and drying the TiO2 nanoparticles,
wherein upon contacting the TiO2 nanoparticles with colorectal cancer cells a cell viability of the colorectal cancer cells is reduced to 20% of a cell viability of a control group of colorectal cancer cells not contacted with the TiO2 nanoparticles,
wherein an amount of the TiO2 nanoparticles in the contacting with the colorectal cancer cells is 0.5 μg per milliliter of a cell culture of the colorectal cancer cells,
wherein upon contacting the TiO2 nanoparticles with the colorectal cancer cells a cell nuclei of the colorectal cancer cells undergo nuclear condensation thereby killing a majority of the colorectal cancer cells,
wherein the TiO2 nanoparticles contain components of the F. pinicola as evident by Fourier transform infrared (FT-IR) spectroscopy absorption peaks at 1414 cm−1, and 1000 cm−1 corresponding to aliphatic C—N, and aromatic C═N bands, respectively,
wherein the TiO2 nanoparticles have a further FT-IR spectroscopy absorption peak at 3439 cm−1 corresponding to —OH, and
wherein the TiO2 nanoparticles have a broadest dimension ranging from 80 to 120 nm.