US 12,220,235 B2
Analyte sensors and sensing methods for dual detection of glucose and ethanol
Tianmei Ouyang, Fremont, CA (US); Benjamin J. Feldman, Berkeley, CA (US); Hyun Cho, Berkeley, CA (US); Lam N. Tran, Dublin, CA (US); Stephen Oja, Alameda, CA (US); and Namvar Kiaie, San Jose, CA (US)
Assigned to Abbott Diabetes Care Inc., Alameda, CA (US)
Filed by Abbott Diabetes Care Inc., Alameda, CA (US)
Filed on Jan. 28, 2020, as Appl. No. 16/774,909.
Claims priority of provisional application 62/884,841, filed on Aug. 9, 2019.
Claims priority of provisional application 62/797,566, filed on Jan. 28, 2019.
Prior Publication US 2020/0237277 A1, Jul. 30, 2020
Int. Cl. A61B 5/1486 (2006.01); A61B 5/00 (2006.01); A61B 5/145 (2006.01); B60K 28/06 (2006.01); C12Q 1/30 (2006.01); C12Q 1/32 (2006.01)
CPC A61B 5/14865 (2013.01) [A61B 5/14532 (2013.01); A61B 5/4845 (2013.01); B60K 28/06 (2013.01); C12Q 1/30 (2013.01); C12Q 1/32 (2013.01); B60K 28/063 (2013.01); B60K 28/066 (2013.01)] 12 Claims
OG exemplary drawing
 
1. An analyte sensor comprising:
a) a working electrode;
b) a glucose-responsive active area disposed upon a surface of the working electrode, the glucose-responsive active area comprising a glucose-responsive active enzyme capable of generating a first signal at the working electrode proportional to a glucose concentration;
c) an ethanol-responsive active area comprising:
i) a first active area disposed directly upon the surface of the working electrode spaced apart from the glucose-responsive active area, the first active area comprising xanthine oxidase and an electron transfer agent, in amounts sufficient to react with acetaldehyde to generate a second signal at the working electrode proportional to an alcohol concentration; and
ii) a second active area isolated from the working electrode, the second active area comprising glucose oxidase and catalase in amounts sufficient to form acetaldehyde upon exposure to ethanol;
d) a first membrane permeable to acetaldehyde disposed upon the first active area; and
e) a second membrane permeable to glucose and ethanol disposed upon the glucose-responsive active area and the second active area,
wherein the second active area is disposed directly upon the first membrane, and
wherein the sensor is configured to be partially inserted into a user's skin.