	US 12,219,960 B2
	Methods and compositions for improving soybean yield
Marcus Jones, Ballwin, MO (US); and Gregg Bogosian, Clarkson Valley, MO (US)
Assigned to NewLeaf Symbiotics, Inc., St. Louis, MO (US)
Filed by NewLeaf Symbiotics, Inc., St. Louis, MO (US)
Filed on Mar. 31, 2021, as Appl. No. 17/219,794.
Application 17/219,794 is a division of application No. 15/100,947, granted, now 10,993,443, previously published as PCT/US2014/068660, filed on Dec. 4, 2014.
Claims priority of provisional application 61/911,698, filed on Dec. 4, 2013.
Prior Publication US 2021/0321622 A1, Oct. 21, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 25/00 (2006.01); A01N 63/00 (2020.01); A01N 63/20 (2020.01)

CPC A01N 63/20 (2020.01) [A01N 25/00 (2013.01); A01N 63/00 (2013.01); Y02A 40/10 (2018.01)]

16 Claims

1. A method for improving soybean plant yield wherein said method comprises:

1) coating or partially coating a soybean plant seed with:

i) a composition comprising an essentially dry fermentation product having about 5% or less water content, wherein said fermentation product comprises a Methylobacterium of NLS0064, deposited as NRRL B-50938; and

ii) an agriculturally acceptable excipient selected from the group consisting of woodflours, clays, activated carbon, diatomaceous earth, fine-grain inorganic solids and calcium carbonate; and/or

an agriculturally acceptable adjuvant selected from the group consisting of polyvinyl acetates, polyvinyl acetate copolymers, hydrolyzed polyvinyl acetates, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl alcohols, polyvinyl alcohol copolymers, polyvinyl methyl ether, polyvinyl methyl ether-maleic anhydride copolymer, waxes, latex polymers, celluloses hydroxy methylcelluloses, hydroxypropylcellulose, hydroxymethylpropylcelluloses, polyvinyl pyrrolidones, alginates, dextrins, malto-dextrins, polysaccharides, fats, oils, proteins, karaya gum, jaguar gum, tragacanth gum, polysaccharide gums, mucilage, gum arabics, shellacs, vinylidene chloride polymers and copolymers, soybean-based protein polymers and copolymers, lignosulfonates, acrylic copolymers, starches, polyvinylacrylates, zeins, gelatin, carboxymethylcellulose, chitosan, polyethylene oxide, acrylamide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylamide monomers, alginate, ethylcellulose, polychloroprene and syrups or mixtures thereof;

2) growing a soybean plant from said soybean plant seed; and

3) harvesting at least one soybean plant part from said soybean plant, wherein the Methylobacterium provides for increased yield of said soybean plant part in comparison to yield of said soybean plant part from a soybean plant grown from a soybean seed not treated with the Methylobacterium of NLS0064.