US 12,219,939 B2
Pathogen-resistant animals having modified CD163 genes
Randall S. Prather, Rocheport, MO (US); Kevin D. Wells, Columbia, MO (US); and Kristin M. Whitworth, Columbia, MO (US)
Assigned to The Curators of the University of Missouri, Columbia, MO (US)
Filed by The Curators of the University of Missouri, Columbia, MO (US)
Filed on Sep. 29, 2020, as Appl. No. 17/036,522.
Application 17/036,522 is a continuation of application No. 15/750,633, granted, now 10,827,730, previously published as PCT/US2016/043467, filed on Jul. 22, 2016.
Claims priority of provisional application 62/202,145, filed on Aug. 6, 2015.
Prior Publication US 2021/0112790 A1, Apr. 22, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/0276 (2024.01); A01K 67/02 (2006.01); C07K 14/705 (2006.01); C12N 5/075 (2010.01); C12N 5/076 (2010.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01); C12N 15/877 (2010.01)
CPC A01K 67/0276 (2013.01) [C07K 14/70596 (2013.01); C12N 5/0609 (2013.01); C12N 5/061 (2013.01); C12N 5/10 (2013.01); C12N 15/09 (2013.01); C12N 15/8509 (2013.01); C12N 15/8778 (2013.01); A01K 67/02 (2013.01); A01K 2217/075 (2013.01); A01K 2227/108 (2013.01); A01K 2267/02 (2013.01); C12N 2310/20 (2017.05); C12N 2770/10011 (2013.01)] 18 Claims
 
1. A porcine animal or offspring thereof comprising a deletion in a gene encoding a CD163 protein, wherein the deletion comprises:
(a) an in-frame deletion in exon 7 of the gene encoding the CD163 protein selected from the group consisting of:
a 123 base pair deletion from nucleotide 3,024 to nucleotide 3,146 as compared to reference sequence SEQ ID NO: 47; and
a 132 base pair deletion from nucleotide 3,030 to nucleotide 3,161 as compared to reference sequence SEQ ID NO: 47;
(b) a deletion of exons 7 and 8 of the gene encoding the CD163 protein selected from the group consisting of:
a 1373 base pair deletion from nucleotide 2,724 to nucleotide 4,096 as compared to reference sequence SEQ ID NO: 47; and
a 1720 base pair deletion from nucleotide 2,440 to nucleotide 4,160 as compared to reference sequence SEQ ID NO: 47; or
(c) a deletion in exon 7 of the gene encoding the CD163 protein selected from the group consisting of:
a 124 base pair deletion from nucleotide 3,024 to nucleotide 3,147 as compared to reference sequence SEQ ID NO: 47;
a 130 base pair deletion from nucleotide 3,030 to nucleotide 3,159 as compared to reference sequence SEQ ID NO: 47;
and
a 28 base pair deletion from nucleotide 3,145 to nucleotide 3,172 as compared to reference sequence SEQ ID NO: 47.