US 12,217,403 B2
Methods and systems to correct crosstalk in illumination emitted from reaction sites
Mohsen Rezaei, Brown Deer, WI (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Jun. 27, 2022, as Appl. No. 17/849,857.
Claims priority of provisional application 63/216,125, filed on Jun. 29, 2021.
Prior Publication US 2022/0414839 A1, Dec. 29, 2022
Int. Cl. G06K 9/00 (2022.01); A61K 35/12 (2015.01); G01N 21/64 (2006.01); G06T 5/00 (2024.01); G06T 5/50 (2006.01); G06T 5/73 (2024.01)
CPC G06T 5/73 (2024.01) [G01N 21/6428 (2013.01); G01N 21/6454 (2013.01); G06T 5/50 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method comprising:
capturing, at each of a set of light sensors, a captured illumination value representing photons detected by that light sensor, wherein:
the set of light sensors is disposed in a two dimensional pattern in which each light sensor from the set of light sensors is adjacent to at least two other light sensors from the set of light sensors; and
each light sensor from the set of light sensors has a corresponding reaction site from a set of reaction sites;
determining a generative function, wherein:
the generative function is determined based on one or more point spread functions (PSFs), each modeling how illumination emitted by a reaction site is detected by both a corresponding light sensor for that reaction site as well as surrounding light sensors; and
the generative function provides a mapping of illumination emitted from the set of reaction sites to photons detected by the set of light sensors;
determining a set of sharpening kernels based on the generative function; and
obtaining a set of crosstalk corrected illumination values based on, for each light sensor from the set of light sensors, applying a corresponding sharpening kernel from the set of sharpening kernels to a set of captured illumination values comprising:
the captured illumination value representing photons detected by that light sensor; and
captured illumination values representing photons detected by a plurality of light sensors, wherein the plurality of light sensors comprises each light sensor which is adjacent to that light sensor;
wherein:
each sharpening kernel from the set of sharpening kernels is a square matrix having a first side length;
the generative function comprises a set of generative matrices, each of which is a square matrix having a second side length;
each of the one or more point spread functions is a square matrix having a third side length;
each generative matrix from the set of generative matrices has a corresponding point spread function;
the second side length is equal to the square of the first side length; and
the first side length and the third side length are both odd.