US 12,216,741 B2
Inter-cluster intensity variation correction and base calling
Eric Jon Ojard, San Francisco, CA (US); Abde Ali Hunaid Kagalwalla, San Diego, CA (US); Rami Mehio, San Diego, CA (US); Nitin Udpa, San Diego, CA (US); Gavin Derek Parnaby, Laguna Niguel, CA (US); and John S. Vieceli, Encinitas, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Nov. 3, 2023, as Appl. No. 18/501,904.
Application 18/501,904 is a continuation of application No. 18/154,603, filed on Jan. 13, 2023, granted, now 11,853,396.
Application 18/154,603 is a continuation of application No. 17/752,789, filed on May 24, 2022, granted, now 11,593,595, issued on Feb. 28, 2023.
Application 17/752,789 is a continuation of application No. 17/510,285, filed on Oct. 25, 2021, granted, now 11,361,194, issued on Jun. 14, 2022.
Claims priority of provisional application 63/106,256, filed on Oct. 27, 2020.
Prior Publication US 2024/0220584 A1, Jul. 4, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G06T 7/33 (2017.01); G06F 18/2135 (2023.01); G06F 18/2411 (2023.01); G06T 7/187 (2017.01); G06V 10/50 (2022.01)
CPC G06F 18/2411 (2023.01) [G06F 18/21355 (2023.01); G06T 7/187 (2017.01); G06V 10/507 (2022.01)] 20 Claims
OG exemplary drawing
 
1. A system comprising at least one processor coupled to memory, the memory loaded with computer instructions that, when executed by the at least one processor, implement actions comprising:
accessing, for a target cluster, current intensities registered from the target cluster and other clusters for a current sequencing cycle;
determining a set of current accumulated intensity correction parameters for the current sequencing cycle based on the current intensities;
determining, for the target cluster and based on the set of current accumulated intensity correction parameters, a current amplification coefficient;
correcting next intensities registered for a next sequencing cycle based on the current amplification coefficient; and
determining a base call for the target cluster at the next sequencing cycle based on the corrected next intensities.