US 12,216,118 B2
System for determining validity of a lateral flow test result
Claus Dallerup Rasmussen, Tumba (SE); and Jonas Trier Hald, Tumba (SE)
Assigned to DeLaval Holding AB, Tumba (SE)
Appl. No. 17/619,110
Filed by DELAVAL HOLDING AB, Tumba (SE)
PCT Filed Jun. 9, 2020, PCT No. PCT/SE2020/050586
§ 371(c)(1), (2) Date Dec. 14, 2021,
PCT Pub. No. WO2020/251460, PCT Pub. Date Dec. 17, 2020.
Claims priority of application No. 1950719-3 (SE), filed on Jun. 14, 2019.
Prior Publication US 2022/0276242 A1, Sep. 1, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 33/53 (2006.01); G01N 33/74 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 33/5302 (2013.01); G01N 33/74 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A system (10) for determining validity of a result of a lateral flow test, comprising:
a lateral flow stick configured to indicate progesterone in a milk sample of an animal (100) by a lateral flow test, said lateral flow stick comprised of:
a sample pad (310), configured to receive the milk sample,
a conjugate pad (315) comprising antibody treated gold particles which disperse into the milk sample when the milk sample is absorbed from the sample pad (310), and
a porous membrane (320) that receives a capillary flow of the milk sample from the conjugate pad (315), said porous membrane (320) comprising:
a test line (360) treated with a progesterone reference which binds to said antibody treated gold particles of the milk sample and thereby causes the test line (360) to change colour tint when exposed to milk having a progesterone level lower than a threshold limit, and
a control line (370) treated with an antibody reference which binds to said antibody treated gold particles of the milk sample regardless of the progesterone level of the milk and thereby causes the control line (370) to change colour tint when exposed to milk containing said antibody treated gold particles;
a sensor (210) configured to capture an image of the control line (370) of the lateral flow stick when the milk sample has been absorbed by the porous membrane (320); and
a control unit (150) configured to:
obtain a reference colour tint (380) of the control line (370),
determine a current colour tint of the control line (370), based on the image captured by the sensor (210) during the lateral flow test,
carry out a comparison of the reference colour tint (380) of the control line (370) with the current colour tint of the control line (370), and
indicate validity of the lateral flow test on the lateral flow stick, based on said comparison.