US 12,216,117 B2
Reagent storage in an assay device
Sudeep Kumar, Hackettstown, NJ (US); George Sigal, Rockville, MD (US); and Michael Tsionsky, Derwood, MD (US)
Assigned to Meso Scale Technologies, LLC., Rockville, MD (US)
Filed by Meso Scale Technologies, LLC., Rockville, MD (US)
Filed on Dec. 16, 2021, as Appl. No. 17/553,441.
Application 16/815,598 is a division of application No. 15/206,656, filed on Jul. 11, 2016, granted, now 10,627,399, issued on Apr. 21, 2020.
Application 17/553,441 is a continuation of application No. 16/815,598, filed on Mar. 11, 2020, granted, now 11,231,417.
Application 15/206,656 is a continuation of application No. 13/272,350, filed on Oct. 13, 2011, abandoned.
Claims priority of provisional application 61/455,112, filed on Oct. 14, 2010.
Prior Publication US 2022/0252590 A1, Aug. 11, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); C12Q 1/6823 (2018.01); C12Q 1/6832 (2018.01); C12Q 1/6834 (2018.01); G01N 33/53 (2006.01)
CPC G01N 33/54386 (2013.01) [C12Q 1/6823 (2013.01); C12Q 1/6832 (2013.01); C12Q 1/6834 (2013.01); G01N 33/5306 (2013.01); G01N 2446/00 (2013.01); G01N 2458/30 (2013.01)] 54 Claims
 
1. An assay device comprising one or more reagent spaces and one or more use zones:
(a) said one or more reagent spaces comprise a storage zone comprising more than one storage surface to which two or more surface-reagent complexes are bound and thereby confined to said storage zone, said two or more surface-reagent complexes comprising a first surface-reagent complex and a second surface-reagent complex, said first surface-reagent complex comprising
(i) a first reagent linked to a first targeting agent;
(ii) a first surface linked to a second targeting agent:
and said second surface-reagent complex comprising:
(iii) a second reagent linked to a third targeting agent; and
(iv) a second surface linked to a fourth targeting agent,
wherein said first reagent and said first surface and said second reagent and said second surface are linked, respectively, in said first and second surface-reagent complexes, via a selective releasable binding interaction between said first and second targeting agents and said third and fourth targeting agents, respectively, wherein said first reagent is released from said first surface-reagent complex by a first set of conditions that differs from a second set of conditions used to release said second reagent from said second surface-reagent complex; and
(b) said one or more use zones are configured to use at least one of said first and second reagents in an assay for an analyte of interest in a sample.