US 12,216,116 B2
Magnetic separation of biological entities from fluid sample
Sasank Vemulapati, Ithaca, NY (US); and David Erickson, Ithaca, NY (US)
Assigned to CORNELL UNIVERSITY, Ithaca, NY (US)
Filed by CORNELL UNIVERSITY, Ithaca, NY (US)
Filed on Jan. 19, 2022, as Appl. No. 17/579,528.
Application 17/579,528 is a continuation of application No. 17/261,855, granted, now 11,262,352, previously published as PCT/US2019/042887, filed on Jul. 22, 2019.
Claims priority of provisional application 62/701,557, filed on Jul. 20, 2018.
Prior Publication US 2022/0146503 A1, May 12, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 1/40 (2006.01); G01N 33/569 (2006.01)
CPC G01N 33/54326 (2013.01) [G01N 1/4077 (2013.01); G01N 33/56966 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A system for use in magnetic separation of a target biological entity from a blood sample, said system comprising:
a vessel comprising a tube or a container for containing the blood sample during operation;
a plurality of magnetic beads each functionalized to bind to the target biological entity;
an aggregation agent configured to induce clumping of a plurality of target biological entities to form aggregates of the target biological entities, wherein the aggregation agent has a concentration of about 3 mg/mL to about 4 mg/mL; and
a magnetic source configured to generate a magnetic field within the vessel to separate the plurality of magnetic beads having bound aggregates of the target biological entities from the blood sample, such that the target biological entities are substantially captured and depleted to separate greater than 99.9% of the target biological entity.