US 12,216,113 B2
Method for preparing analysis sample, analysis method, and kit for preparing analysis sample
Takashi Nishikaze, Kyoto (JP); Hisatoshi Hanamatsu, Sapporo (JP); Jun-ichi Furukawa, Sapporo (JP); and Ikuko Yokota, Sapporo (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Filed by SHIMADZU CORPORATION, Kyoto (JP)
Filed on Oct. 19, 2020, as Appl. No. 17/073,608.
Claims priority of application No. 2019-192037 (JP), filed on Oct. 21, 2019.
Prior Publication US 2021/0164989 A1, Jun. 3, 2021
Int. Cl. G01N 33/50 (2006.01); C07H 1/00 (2006.01)
CPC G01N 33/50 (2013.01) [C07H 1/00 (2013.01); G01N 2400/00 (2013.01); G01N 2560/00 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method for preparing an analysis sample from a sample containing a glycan, the method comprising:
1) Performing esterification reaction on the sample that subjects at least a part of a sialic acid included in the glycan to esterification other than lactonization; and
2) Performing amidation reaction that converts an esterified form of the sialic acid modified through the esterification other than lactonization according to step 1 into an amidated form through contacting the at least a part of the sample after the esterification reaction according to step 1 with an amidation reaction solution containing at least one compound selected from the group consisting of ammonia, amines, hydrazine, hydrazine derivatives, and hydroxyamine, and salts thereof to be reacted with the sialic acid modified through the esterification.