US 12,216,101 B2
Chromatography apparatus and method
Ana Stern, Uppsala (SE)
Assigned to Cytiva Sweden AB, Uppsala (SE)
Appl. No. 17/620,035
Filed by Cytiva Sweden AB, Uppsala (SE)
PCT Filed Jun. 16, 2020, PCT No. PCT/EP2020/066646
§ 371(c)(1), (2) Date Dec. 16, 2021,
PCT Pub. No. WO2020/260074, PCT Pub. Date Dec. 30, 2020.
Claims priority of application No. 1909275 (GB), filed on Jun. 27, 2019.
Prior Publication US 2022/0252561 A1, Aug. 11, 2022
Int. Cl. G01N 30/86 (2006.01); B01D 15/34 (2006.01)
CPC G01N 30/8675 (2013.01) [B01D 15/34 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A computer implemented method performed by a chromatography apparatus configured to separate molecules, having varying size, from an eluent, the method comprising:
obtaining reference data for a chromatography column of the chromatography apparatus, wherein the reference data is indicative of elution characteristics for a set of molecular sizes,
obtaining an elution progress measure and a corresponding quantitative measure indicative of a concentration of molecules in an eluate of the chromatography apparatus,
estimating a size measure indicative of molecular size of the molecules in the eluate,
rendering a representation indicative of the quantitative measure and the size measure,
controlling a display to display the representation to a user of the chromatography apparatus,
wherein the molecular size is estimated using a predetermined relation, comprised in the reference data, and the elution progress measure,
further wherein the predetermined relation is indicating a curve, and the predetermined relation is obtained by performing curve fitting of value pairs comprising a gel-phase distribution coefficient (Kav) and corresponding molecular size.