	US 12,216,089 B2
	Processing system for processing specimens using acoustic energy
David Chafin, Tucson, AZ (US); Michael Otter, Tucson, AZ (US); Abbey Pierson, Tucson, AZ (US); and Jefferson Curtis Taft, Sahuarita, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Feb. 9, 2023, as Appl. No. 18/107,951.
Application 18/107,951 is a continuation of application No. 16/287,827, filed on Feb. 27, 2019, granted, now 11,614,426.
Application 16/287,827 is a continuation of application No. 13/582,705, granted, now 10,267,769, issued on Apr. 23, 2019, previously published as PCT/US2011/027284, filed on Mar. 4, 2011.
Claims priority of provisional application 61/310,653, filed on Mar. 4, 2010.
Prior Publication US 2023/0184721 A1, Jun. 15, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 29/07 (2006.01); G01N 1/30 (2006.01); G01N 29/024 (2006.01); G01N 29/44 (2006.01); G01N 33/483 (2006.01)

CPC G01N 29/07 (2013.01) [G01N 1/30 (2013.01); G01N 29/024 (2013.01); G01N 29/4427 (2013.01); G01N 33/4833 (2013.01); G01N 2291/02466 (2013.01); G01N 2291/02475 (2013.01)]

15 Claims

1. A method of processing a tissue sample comprising:

(i) immersing the tissue sample in a fixative solution having a first temperature ranging from 0° C. to 10° C. for a first time period sufficient to diffuse the fixative solution into an interior region of the tissue sample without substantially forming cross-links within the tissue sample; and

(ii) continuing immersion of the tissue sample in the fixative solution for a second period of time sufficient to cause cross-linking within the tissue sample,

wherein the tissue sample is not exposed to ultrasonic or electromagnetic irradiation during either (i) the immersion of the tissue sample in the fixative solution, or (ii) the continuing immersion of the tissue sample in the fixative solution.