US 12,216,074 B2
Biosensors and methods for determining analyte concentration in the kinetic potential region of redox mediators
Thomas Seidl, Portland, OR (US); William Kenneth Ward, Portland, OR (US); and Huan-Ping Wu, Portland, OR (US)
Assigned to Pacific Diabetes Technologies, Inc., Portland, OR (US)
Filed by Pacific Diabetes Technologies, Inc., Portland, OR (US)
Filed on Sep. 29, 2022, as Appl. No. 17/956,185.
Claims priority of provisional application 63/251,191, filed on Oct. 1, 2021.
Prior Publication US 2024/0094156 A1, Mar. 21, 2024
Int. Cl. G01N 27/26 (2006.01); A61B 5/145 (2006.01); A61B 5/1486 (2006.01)
CPC G01N 27/26 (2013.01) [A61B 5/14532 (2013.01); A61B 5/1486 (2013.01)] 62 Claims
OG exemplary drawing
 
1. A method for determining an analyte concentration in a sample, the method comprising:
applying a first and second calibration input potentials to a subcutaneously inserted test sensor, where the first and second calibration input potentials are within a kinetic potential region of a redox mediator of the test subcutaneously inserted test sensor;
measuring output currents responsive to the first and the second calibration input potentials from the subcutaneously inserted test sensor;
determining a regression sensitivity relationship from the measured output currents responsive to the first and the second calibration input potentials;
determining a first analysis input potential within the kinetic potential region of the redox mediator from the regression sensitivity relationship;
applying the first analysis input potential to the subcutaneously inserted test sensor;
modifying a first redox state to a second redox state of the redox mediator, where a concentration of the first redox state of the redox mediator is responsive to an analyte concentration in a sample;
measuring output currents responsive to the first analysis input potential from the subcutaneously inserted test sensor;
determining the analyte concentration of the sample in response to the measured output currents responsive to the first analysis input potential; and
reporting the determined analyte concentration to a user.