US 12,216,060 B2
Quantifying color changes in chemical test pads induced by different concentrations of analytes under different lighting conditions
Bernard Burg, Menlo Park, CA (US); Martin Zizi, Enines (BE); Aaron Alexander Rowe, San Francisco, CA (US); Anthony Smart, Costa Mesa, CA (US); and Walter De Brouwer, Los Altos, CA (US)
Assigned to HEALTHY.IO LTD., Tel Aviv-Jaffa (IL)
Filed by HEALTHY.IO LTD., Tel Aviv-Jaffa (IL)
Filed on Sep. 20, 2023, as Appl. No. 18/370,669.
Application 18/370,669 is a continuation of application No. 17/467,863, filed on Sep. 7, 2021, granted, now 11,802,839.
Application 17/467,863 is a continuation of application No. 16/297,630, filed on Mar. 9, 2019, granted, now 11,131,633, issued on Sep. 28, 2021.
Application 16/297,630 is a continuation of application No. 15/055,643, filed on Feb. 29, 2016, granted, now 10,267,743, issued on Apr. 23, 2019.
Application 15/055,643 is a continuation of application No. 14/633,513, filed on Feb. 27, 2015, granted, now 9,285,323, issued on Mar. 15, 2016.
Application 14/633,513 is a continuation in part of application No. 14/419,939, granted, now 9,311,520, issued on Apr. 12, 2016, previously published as PCT/US2013/035397, filed on Apr. 5, 2013.
Claims priority of provisional application 61/948,356, filed on Mar. 5, 2014.
Claims priority of provisional application 61/680,842, filed on Aug. 8, 2012.
Prior Publication US 2024/0011912 A1, Jan. 11, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/78 (2006.01); G01N 21/77 (2006.01); G01N 21/79 (2006.01); G01N 33/48 (2006.01)
CPC G01N 21/78 (2013.01) [G01N 21/79 (2013.01); G01N 2021/7759 (2013.01); G01N 33/48 (2013.01); G01N 2201/0221 (2013.01); G01N 2201/12 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for quantifying color change of at least one test medium on a diagnostic instrument, the method comprising:
obtaining a digital image of at least a portion of the diagnostic instrument that has been exposed to a biological sample, the diagnostic instrument comprising at least one color reference including a plurality of reference samples of different colors and at least one test medium containing a reagent that changes color in response to a concentration of a particular analyte in the biological sample;
identifying at least one reference sample of the plurality of reference samples for the at least one test medium in the diagnostic instrument;
determining a dominant camera-captured color of the at least one reference sample and a dominant camera-captured color of the at least one test medium;
estimating lighting conditions under which the digital image is obtained;
correcting the dominant camera-captured color of the at least one test medium, in response to a color correction factor derived at least in part from the dominant camera-captured color of the at least one reference sample, to determine a corrected test medium color; and
determining a test result including an analyte concentration of the biological sample based on the corrected test medium color.