US 12,216,034 B2
Methods to increase sensitivity of LC/MS analysis
Paul Rainville, Princeton, MA (US); and Kerri Smith, Marlborough, MA (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Mar. 13, 2024, as Appl. No. 18/604,005.
Application 18/604,005 is a continuation of application No. 17/990,029, filed on Nov. 18, 2022, granted, now 11,959,842.
Application 17/990,029 is a continuation of application No. 17/150,793, filed on Jan. 15, 2021, granted, now 11,530,966, issued on Dec. 20, 2022.
Claims priority of provisional application 63/071,750, filed on Aug. 28, 2020.
Claims priority of provisional application 62/962,476, filed on Jan. 17, 2020.
Prior Publication US 2024/0219275 A1, Jul. 4, 2024
Int. Cl. G01N 30/72 (2006.01); G01N 1/40 (2006.01); G01N 30/86 (2006.01); G01N 30/02 (2006.01)
CPC G01N 1/405 (2013.01) [G01N 30/7233 (2013.01); G01N 30/8631 (2013.01); G01N 2030/027 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method of separating a sample comprising:
providing a chromatographic column having a metal flow path with an alkylsilyl coating on fluid exposed surfaces, the metal flow path with the alkylsilyl coating preventing the sample from binding to underlying metal surfaces;
injecting the sample onto the chromatographic column, wherein the sample comprises 2-hydroxyglutaric acid;
flowing the sample through the chromatographic column; and
separating the sample, wherein the alkylsilyl coating on the metal flow path of the chromatographic column increases a chromatographic peak area of the 2-hydroxyglutaric acid in the sample in comparison to an uncoated chromatographic column having fluid exposed metal surfaces by 3.5× or greater.