US 12,216,033 B2
Contact-type patch, staining method using the same, and manufacturing method thereof
Dongyoung Lee, Yongin-si (KR); Kyunghwan Kim, Yongin-si (KR); Youngmin Shin, Yongin-si (KR); Hyunjeong Yang, Seongnam-si (KR); and Chanyang Lim, Seongnam-si (KR)
Assigned to NOUL CO, LTD., Yongin-si (KR)
Filed by NOUL CO, LTD., Yongin-si (KR)
Filed on Jul. 13, 2023, as Appl. No. 18/351,796.
Application 16/417,419 is a division of application No. 15/498,343, filed on Apr. 26, 2017, granted, now 10,345,204, issued on Jul. 9, 2019.
Application 18/351,796 is a continuation of application No. 16/417,419, filed on May 20, 2019, granted, now 11,740,162.
Application 15/498,343 is a continuation of application No. 15/206,247, filed on Jul. 9, 2016, granted, now 10,371,610, issued on Aug. 6, 2019.
Claims priority of provisional application 62/298,959, filed on Feb. 23, 2016.
Claims priority of application No. 10-2016-0069936 (KR), filed on Jun. 4, 2016; application No. 10-2016-0069937 (KR), filed on Jun. 4, 2016; and application No. 10-2016-0069938 (KR), filed on Jun. 4, 2016.
Prior Publication US 2023/0366792 A1, Nov. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 1/31 (2006.01); B01L 3/00 (2006.01); C07K 16/30 (2006.01); C12Q 1/6844 (2018.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01); C12Q 1/70 (2006.01); G01N 1/30 (2006.01); G01N 15/06 (2006.01); G01N 15/14 (2006.01); G01N 21/77 (2006.01); G01N 33/483 (2006.01); G01N 33/49 (2006.01); G01N 33/50 (2006.01); G01N 33/52 (2006.01); G01N 33/53 (2006.01); G01N 33/533 (2006.01); G01N 33/574 (2006.01); G01N 33/60 (2006.01); G06T 7/00 (2017.01); B01L 7/00 (2006.01); G01N 15/01 (2024.01); G01N 15/075 (2024.01)
CPC G01N 1/312 (2013.01) [B01L 3/00 (2013.01); C07K 16/3061 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/686 (2013.01); C12Q 1/701 (2013.01); G01N 1/30 (2013.01); G01N 1/31 (2013.01); G01N 15/06 (2013.01); G01N 15/14 (2013.01); G01N 21/77 (2013.01); G01N 33/4833 (2013.01); G01N 33/49 (2013.01); G01N 33/5082 (2013.01); G01N 33/52 (2013.01); G01N 33/5304 (2013.01); G01N 33/533 (2013.01); G01N 33/574 (2013.01); G01N 33/60 (2013.01); G06T 7/0012 (2013.01); G06T 7/0014 (2013.01); B01L 3/505 (2013.01); B01L 7/52 (2013.01); G01N 2001/302 (2013.01); G01N 15/01 (2024.01); G01N 15/075 (2024.01); G01N 2021/7723 (2013.01); G01N 2021/7786 (2013.01)] 7 Claims
 
1. A method of staining a specimen using a gel-phase contact-type staining patch, the method comprising:
staining a specimen fixed on a slide with a staining reagent,
wherein, the staining step comprises,
contacting a surface of the gel-phase contact-type staining patch comprising a staining reagent configured to react with the specimen and a buffer solution to the specimen fixed on the slide; and
separating the gel-phase contact-type staining patch from the specimen.