US 12,216,032 B2
Device and method for the dissociation of tissue
Christian Reis, Römerberg (DE); Stefan Scheuermann, Mannheim (DE); Armin Schäfer, Mannheim (DE); and Daniel Edinger, Spechbach (DE)
Assigned to Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., Munich (DE)
Appl. No. 15/733,424
Filed by Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., Munich (DE)
PCT Filed Jan. 24, 2018, PCT No. PCT/EP2018/051701
§ 371(c)(1), (2) Date Sep. 29, 2020,
PCT Pub. No. WO2019/145029, PCT Pub. Date Aug. 1, 2019.
Prior Publication US 2021/0140856 A1, May 13, 2021
Int. Cl. G01N 1/00 (2006.01); B02C 18/06 (2006.01); B02C 18/10 (2006.01); B02C 19/00 (2006.01); C12M 3/08 (2006.01); G01N 1/28 (2006.01); G01N 1/40 (2006.01)
CPC G01N 1/286 (2013.01) [B02C 18/062 (2013.01); B02C 18/10 (2013.01); B02C 19/0056 (2013.01); C12M 3/08 (2013.01); G01N 1/4077 (2013.01); G01N 2001/2866 (2013.01); G01N 2001/4088 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A device for dissociation of tissue, the device comprising:
at least one dissociation unit for receiving a tissue sample; and
at least one cell strainer, wherein the at least one dissociation unit is disposed at least partially in the at least one cell strainer, and wherein the at least one dissociation unit includes:
at least one vessel for receiving the tissue sample; and
a grinder disposed in the at least one vessel, wherein the grinder includes at least one first row of teeth with a plurality of teeth and at least one second row of teeth with a second plurality of teeth, the at least one first row of teeth being disposed on a base of the at least one vessel and the at least one second row of teeth being disposed on a cover of the at least one vessel, the at least one first row of teeth being movable relative to the at least one second row of teeth so that the teeth of the at least one first row of teeth move past the teeth of the at least one second row of teeth, wherein one of the at least one first row of teeth and one of the at least one second row of teeth are disposed at such a spacing respectively adjacent to each other so that the teeth of the respectively adjacent rows of teeth, when they are moved past with respect to each other, effect a dissociation of a part of the tissue sample in a spacing region by their minimum spacing relative to each other.