US 12,215,394 B2
Molecular marker nicotine associated SNP 1 for identifying high or low nicotine content of tobacco and its kit as well as use thereof
He Xie, Kunming (CN); Ge Bai, Kunming (CN); Yong Li, Kunming (CN); Aiguo Yang, Kunming (CN); Tao Pang, Kunming (CN); Dahai Yang, Kunming (CN); Bingguang Xiao, Kunming (CN); Yongping Li, Kunming (CN); Min Ren, Kunming (CN); and Mingliang Fei, Kunming (CN)
Filed by Yunan Academy of Tobacco Agricultural Science, Kunming (CN)
Filed on Nov. 23, 2021, as Appl. No. 17/534,204.
Claims priority of application No. 202111324714.4 (CN), filed on Nov. 10, 2021.
Prior Publication US 2023/0203601 A1, Jun. 29, 2023
Int. Cl. C12Q 1/6895 (2018.01); A01H 1/04 (2006.01); G16B 20/20 (2019.01)
CPC C12Q 1/6895 (2013.01) [A01H 1/04 (2013.01); G16B 20/20 (2019.02)] 6 Claims
 
1. A kit for identifying high or low nicotine content of tobacco, comprising a molecular marker Nicotine Associated SNP 1, wherein the molecular marker Nicotine Associated SNP 1 is a single nucleotide polymorphism SNP site Nitab4.5_0002539:95304 A/G at base No. 95304 of Genomic segment No. 0002539 in tobacco genome version of Nitab v4.5 Genome Scaffolds Edwards2017; the SNP site is located at the 324th nucleotide of the amplicon sequence set forth in SEQ ID NO: 13, which is derived from the tobacco genome using primers set forth in SEQ ID NO:1 and SEQ ID NO:2, and wherein the kit further comprises a pair of specific primers for the molecular marker Nicotine Associated SNP 1 comprising SEQ ID NO:1 and SEQ ID NO:2.