US 12,215,389 B2
Compositions for detecting epigenetic modifications
Manon Van Engeland, Maastricht (NL); Manon Adriaan De Bruine, Maastricht (NL); Arjan Griffioen, Maastricht (NL); Joost Louwagie, Sart-Tilman (BE); Katja Bierau, Sart-Tilman (BE); Gontran Brichard, Sart-Tilman (BE); Gaetan Otto, Sart-Tilman (BE); and Maarten Penning, Sart-Tilman (BE)
Assigned to Exact Sciences Corporation, Madison, WI (US)
Filed by EXACT SCIENCES CORPORATION, Madison, WI (US)
Filed on Sep. 15, 2021, as Appl. No. 17/475,584.
Application 17/475,584 is a division of application No. 16/154,023, filed on Oct. 8, 2018, granted, now 11,149,317.
Application 16/154,023 is a continuation of application No. 15/971,461, filed on May 4, 2018, granted, now 10,808,286, issued on Oct. 20, 2020.
Application 15/971,461 is a continuation of application No. 14/613,574, filed on Feb. 4, 2015, granted, now 9,982,308, issued on May 29, 2018.
Application 14/613,574 is a continuation of application No. 12/522,648, granted, now 8,969,046, issued on Mar. 3, 2015, previously published as PCT/GB2008/000056, filed on Jan. 9, 2008.
Claims priority of provisional application 60/978,261, filed on Oct. 8, 2007.
Claims priority of provisional application 60/960,130, filed on Sep. 17, 2007.
Claims priority of provisional application 60/879,332, filed on Jan. 9, 2007.
Prior Publication US 2022/0064740 A1, Mar. 3, 2022
Int. Cl. C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6886 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 2600/106 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01)] 8 Claims
 
1. A composition comprising a reaction mixture, the reaction mixture comprising:
sample DNA that is treated with a bisulfite reagent that modifies unmethylated DNA to form treated sample DNA, wherein the sample DNA is from a human;
NDRG4-specific primers that hybridize to a target sequence in the treated sample DNA that is within a promoter region of a human NDRG4 gene that corresponds to positions 1-1000 of methylated and bisulfite-treated sequence SEQ ID NO: 524;
amplified DNA obtained by amplifying the treated sample DNA using the NDRG4-specific primers; and
a fluorophore-labeled NDRG4-specific probe hybridizable to a site in the amplified DNA that is within a sequence that corresponds to positions 1-1000 of methylated and bisulfite-treated sequence SEQ ID NO: 524.