US 12,215,383 B2
Polymerase compositions and methods of making and using same
Daniel Mazur, San Diego, CA (US); Eileen Tozer, San Diego, CA (US); Sihong Chen, Vista, CA (US); Peter Vander Horn, Encinitas, CA (US); and Tommie Lincecum, Carlsbad, CA (US)
Assigned to Life Technologies Corporation, Carlsbad, CA (US)
Filed by Life Technologies Corporation, Carlsbad, CA (US)
Filed on Apr. 20, 2022, as Appl. No. 17/725,369.
Application 15/961,206 is a division of application No. 14/970,818, filed on Dec. 16, 2015, granted, now 9,976,178, issued on May 22, 2018.
Application 17/725,369 is a continuation of application No. 16/947,742, filed on Aug. 14, 2020, granted, now 11,319,584.
Application 16/947,742 is a continuation of application No. 15/961,206, filed on Apr. 24, 2018, granted, now 10,745,747, issued on Aug. 18, 2020.
Claims priority of provisional application 62/092,756, filed on Dec. 16, 2014.
Prior Publication US 2022/0307071 A1, Sep. 29, 2022
Int. Cl. C12N 9/12 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/686 (2013.01) [C12N 9/1252 (2013.01); C12Q 1/6869 (2013.01); C12Q 2521/101 (2013.01); C12Y 207/07007 (2013.01)] 26 Claims
 
1. A composition comprising:
a modified polypeptide having at least 95% identity to SEQ ID NO: 1, wherein the modified polypeptide exhibits polymerase activity and an improvement relative to a reference polymerase of SEQ ID NO:1 and/or SEQ ID NO:34, in one or more property comprising thermostability and/or at least one sequencing property, and
wherein the modified polypeptide comprises one or more amino acid substitutions selected from A77E, A97V, K240I, L287T, E295F, E295N, and E794C, and
wherein the modified polypeptide comprises the naturally occurring DNA polymerase motifs A and C.