US 12,215,380 B2
Nucleic acid sequence measurement device
Takashi Tadenuma, Tokyo (JP); Yuki Miyauchi, Tokyo (JP); and Tomoyuki Taguchi, Tokyo (JP)
Assigned to Yokogawa Electric Corporation, Tokyo (JP)
Appl. No. 17/291,010
Filed by Yokogawa Electric Corporation, Tokyo (JP)
PCT Filed Oct. 18, 2019, PCT No. PCT/JP2019/041142
§ 371(c)(1), (2) Date May 4, 2021,
PCT Pub. No. WO2020/095657, PCT Pub. Date May 14, 2020.
Claims priority of application No. 2018-211188 (JP), filed on Nov. 9, 2018.
Prior Publication US 2022/0042080 A1, Feb. 10, 2022
Int. Cl. C12Q 1/6818 (2018.01); C12Q 1/6837 (2018.01)
CPC C12Q 1/6818 (2013.01) [C12Q 1/6837 (2013.01)] 15 Claims
 
1. A nucleic acid sequence measurement device that measures a target having a specific nucleic acid sequence by means of hybridization, a sample being a solution containing the target, the nucleic acid sequence measurement device comprising:
a fluorescent probe with a fluorescent molecule added at a predetermined position;
a quenching probe with a quenching molecule added at a predetermined position; and
a substrate having a solid phase surface to which a base end of each of the fluorescent probe and the quenching probe is fixed,
wherein one of the fluorescent probe and the quenching probe has a detection sequence having a nucleic acid sequence complementary to a nucleic acid sequence of the target,
wherein the other one of the fluorescent probe and the quenching probe has a nucleic acid sequence complementary to a nucleic acid sequence of a part of the detection sequence (which will also be referred to as a complementary sequence) and a nucleic acid sequence from a base end of the complementary sequence on a side of the solid phase surface to the other base end is complementary to the part of the detection sequence,
wherein the fluorescent probe or the quenching probe having the detection sequence has a longer nucleic acid sequence than the fluorescent probe or the quenching probe having the complementary sequence,
wherein the base end of the fluorescent probe and the base end of the quenching probe are fixed to the solid phase surface so that a positional relationship in which fluorescence emitted by the fluorescent molecule is quenched by the quenching molecule close to the fluorescent molecule is established, and
wherein the nucleic acid sequence from the base end of the complementary sequence on the side of the solid phase surface to the other base end is complementary to a nucleic acid sequence from a base end of the detection sequence on the side of the solid phase surface to a portion corresponding to the other base end of the complementary sequence.