	US 12,215,371 B2
	Enzymatic production of hexoses
Daniel Joseph Wichelecki, Charlottesville, VA (US)
Assigned to BONUMOSE, INC., Charlottesville, VA (US)
Appl. No. 17/287,240
Filed by BONUMOSE, INC., Charlottesville, VA (US)
PCT Filed Oct. 29, 2019, PCT No. PCT/US2019/058483 § 371(c)(1), (2) Date Apr. 21, 2021, PCT Pub. No. WO2020/092315, PCT Pub. Date May 7, 2020.
Claims priority of provisional application 62/857,543, filed on Jun. 5, 2019.
Claims priority of provisional application 62/752,061, filed on Oct. 29, 2018.
Prior Publication US 2021/0381014 A1, Dec. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/02 (2006.01); C12N 9/10 (2006.01); C12N 9/90 (2006.01)

CPC C12P 19/02 (2013.01) [C12N 9/1051 (2013.01); C12N 9/90 (2013.01); C12Y 204/01001 (2013.01); C12Y 204/01007 (2013.01); C12Y 204/01025 (2013.01); C12Y 504/02002 (2013.01)]

19 Claims

1. An improved process for the enzymatic production of a hexose, wherein the improved process comprises:

a) converting glucose 1-phosphate (G1P) to glucose 6-phosphate (G6P), catalyzed by a higher activity phosphoglucomutase (PGM), wherein the higher activity PGM results in an improved process that, when the hexose is fructose, produces at least 800% more fructose relative to a process to produce fructose under equivalent process conditions in which the conversion of G1P to G6P is catalyzed by a PGM corresponding to Uniprot ID Q68BJ6 from Thermococcus kodakaraensis;

b) converting the G6P to fructose 6-phosphate (F6P), catalyzed by a phosphoglucoisomerase (PGI); and

c) enzymatically converting the F6P to the hexose, catalyzed by an epimerase, an isomerase, a phosphatase, or a combination thereof.