	US 12,215,369 B2
	Method for the in vivo synthesis of 4-hydroxymethylfurfural and derivatives thereof
Paulo Moises Raduan Alexandrino, Campinas (BR); Iuri Estrada Gouvea, Campinas (BR); and Veronica Leite Queiroz, Campinas (BR)
Assigned to BRASKEM S.A., Sao Paulo (BR)
Filed by Braskem S.A., Sao Paulo (BR)
Filed on May 20, 2022, as Appl. No. 17/750,293.
Application 17/750,293 is a continuation of application No. 16/806,728, filed on Mar. 2, 2020, granted, now 11,339,414.
Claims priority of provisional application 62/812,904, filed on Mar. 1, 2019.
Prior Publication US 2022/0298535 A1, Sep. 22, 2022
Int. Cl. C12P 17/04 (2006.01); C07D 307/68 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/08 (2006.01); C12N 9/16 (2006.01); C12N 9/88 (2006.01)

CPC C12P 17/04 (2013.01) [C07D 307/68 (2013.01); C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/0065 (2013.01); C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12Y 101/01 (2013.01); C12Y 102/01 (2013.01); C12Y 101/01001 (2013.01); C12Y 101/03 (2013.01); C12Y 102/01003 (2013.01); C12Y 102/01004 (2013.01); C12Y 102/01005 (2013.01); C12Y 207/01 (2013.01); C12Y 301/03 (2013.01); C12Y 402/03 (2013.01)]

18 Claims

1. A method of producing 2,4-furandicarboxylic acid (2,4-FDCA) from glyceraldehyde 3-phosphate (G3P), the method comprising:

(a) providing G3P in the presence of a methyl phosphate synthase that catalyzes a conversion of G3P to (5-formylfuran-3-yl)methyl phosphate;

(b) providing the (5-formylfuran-3-yl)methyl phosphate from step (a) to a phosphatase that catalyzes a conversion of the (5-formylfuran-3-yl)methyl phosphate to 4-hydroxymethylfurfural (4-HMF); and

(c) providing the 4-HMF from step (b) to an oxidase, a dehydrogenase and/or a peroxygenase that catalyze(s) an oxidation of the 4-HMF to 2,4-FDCA, wherein the oxidation of 4-HMF to 2,4-FDCA, occurs through one or more intermediates selected from the group consisting of: furan-2,4-dicarbaldehyde, 4-(hydroxymethyl) furoic acid, and 4-formylfuran-2-carboxylate;

wherein the phosphatase is a haloacid dehalogenase or comprises an amino acid sequence as set forth in SEQ ID NO: 28, any one of SEQ ID Nos: 40-52, or any one of SEQ ID NOs: 53-68.