	US 12,215,368 B2
	Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Xiang Yi, San Carlos, CA (US); Oscar Alvizo, Fremont, CA (US); Ravi David Garcia, Los Gatos, CA (US); David Entwistle, San Carlos, CA (US); Charlene Ching, San Jose, CA (US); and James Nicholas Riggins, San Francisco, CA (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Appl. No. 17/600,345
Filed by Codexis, Inc., Redwood City, CA (US)
PCT Filed Apr. 23, 2020, PCT No. PCT/US2020/029507 § 371(c)(1), (2) Date Sep. 30, 2021, PCT Pub. No. WO2020/223102, PCT Pub. Date Nov. 5, 2020.
Claims priority of provisional application 62/841,633, filed on May 1, 2019.
Prior Publication US 2022/0213518 A1, Jul. 7, 2022
Int. Cl. C12N 9/06 (2006.01); C12P 13/04 (2006.01)

CPC C12P 13/04 (2013.01) [C12N 9/0028 (2013.01)]

17 Claims

1. An engineered polypeptide having imine reductase activity and comprising an amino acid sequence with at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 4, wherein the tyrosine at the position corresponding to amino acid 222 of SEQ ID NO: 4 is substituted with another amino acid in the sequence of the engineered polypeptide.