US 12,215,359 B2
Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
Xiang Yi, San Carlos, CA (US); Oscar Alvizo, Fremont, CA (US); Ravi David Garcia, Los Gatos, CA (US); David Entwistle, San Carlos, CA (US); Charlene Ching, San Jose, CA (US); James Nicholas Riggins, San Francisco, CA (US); and Nandhitha Subramanian, Cambridge (GB)
Assigned to Codexis, Inc., Redwood City, CA (US)
Appl. No. 17/600,357
Filed by Codexis, Inc., Redwood City, CA (US)
PCT Filed Apr. 23, 2020, PCT No. PCT/US2020/029517
§ 371(c)(1), (2) Date Sep. 30, 2021,
PCT Pub. No. WO2020/223104, PCT Pub. Date Nov. 5, 2020.
Claims priority of provisional application 62/841,666, filed on May 1, 2019.
Prior Publication US 2022/0145268 A1, May 12, 2022
Int. Cl. C12N 9/04 (2006.01); C12N 1/20 (2006.01); C12N 15/72 (2006.01)
CPC C12N 9/0006 (2013.01) [C12N 1/20 (2013.01); C12N 15/72 (2013.01)] 21 Claims
 
1. An engineered glucose dehydrogenase polypeptide comprising a polypeptide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:4, wherein said engineered glucose dehydrogenase polypeptide comprises a substitution at amino acid position 147 in said polypeptide sequence selected from the group consisting of H147A/S/I/T/Q/R, wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:4, and wherein said engineered glucose dehydrogenase polypeptide exhibits improved production of compound (1)

OG Complex Work Unit Chemistry
compared to the engineered glucose dehydrogenase polypeptide of SEQ ID NO:4.