	US 12,215,358 B1
	Coxsackievirus A6 strain CVA6-KM-J33 and use thereof
Longding Liu, Kunming (CN); Li Yu, Kunming (CN); Guorun Jiang, Kunming (CN); Dandan Li, Kunming (CN); Heng Zhao, Kunming (CN); Huiwen Zheng, Kunming (CN); Ying Zhang, Kunming (CN); Yun Liao, Kunming (CN); Haijing Shi, Kunming (CN); Xin Zhao, Kunming (CN); and Heng Li, Kunming (CN)
Assigned to INSTITUTE OF MEDICAL BIOLOGY, CAMS, Kunming (CN)
Filed by INSTITUTE OF MEDICAL BIOLOGY, CAMS, Kunming (CN)
Filed on May 3, 2024, as Appl. No. 18/654,901.
Claims priority of application No. 202311074921.8 (CN), filed on Aug. 24, 2023; and application No. 202311481600.X (CN), filed on Nov. 9, 2023.
Int. Cl. C12N 7/00 (2006.01); C12N 5/071 (2010.01)

CPC C12N 7/00 (2013.01) [C12N 5/0688 (2013.01); C12N 2500/02 (2013.01); C12N 2523/00 (2013.01); C12N 2770/32321 (2013.01); C12N 2770/32351 (2013.01)]

1 Claim

1. A method for propagating and passaging the CVA6 strain CVA6-KM-J33 comprising infecting a human embryonic lung diploid cell with a Coxsackievirus A6 (CVA6) strain CVA6-KM-J33 and culturing the human embryonic lung diploid cell at 37° C. under 5% CO2;

wherein the human embryonic lung diploid cell is a KMB17 human embryonic lung diploid cell; and

wherein the CVA6 strain CVA6-KM-J33 has a deposit number of CCTCC NO. V202384 in the China Center for Type Culture Collection and a VP1 structural protein of the CVA6 strain CVA6-KM-J33 has the amino acid sequence set forth in SEQ ID NO: 10.