US 12,215,357 B2
Apparatus and method for establishment of induced pluripotent stem cells
Katsuaki Deguchi, Otawara (JP)
Assigned to Canon Medical Systems Corporation, Otawara (JP); and Canon Kabushiki Kaisha, Tokyo (JP)
Filed by Canon Medical Systems Corporation, Otawara (JP); and Canon Kabushiki Kaisha, Tokyo (JP)
Filed on Oct. 18, 2021, as Appl. No. 17/503,634.
Claims priority of application No. 2020-175302 (JP), filed on Oct. 19, 2020.
Prior Publication US 2022/0119774 A1, Apr. 21, 2022
Int. Cl. C12N 5/074 (2010.01); C12M 1/00 (2006.01)
CPC C12N 5/0696 (2013.01) [C12M 45/06 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for supplying a mixed liquid containing a mononuclear cell and an inducing factor for inducing reprogramming of the mononuclear cell to a container, the method comprising:
a first step of sending a suspension liquid containing the mononuclear cell to a trap through a first flow channel in a first direction, the trap configured to trap the mononuclear cell contained in the suspension liquid sent in the first step, the trap being arranged in the first flow channel; and
a second step of sending the inducing factor to the trap in a second direction opposite the first direction through the first flow channel, and sending the mononuclear cell trapped by the trap and the inducing factor to the container through a second flow channel branching off from the first flow channel at a branch position upstream of the trap in the first direction.