US 12,215,353 B2
Plated hepatocytes and preparation and uses thereof
Jung Bok Lee, Virginia Beach, VA (US); Angela Murchison, Virginia Beach, VA (US); Edward LeCluyse, Virginia Beach, VA (US); and Jingsong Chen, Virginia Beach, VA (US)
Assigned to LifeNet Health, Virginia Beach, VA (US)
Filed by LifeNet Health, Virginia Beach, VA (US)
Filed on Dec. 5, 2022, as Appl. No. 18/074,837.
Application 18/074,837 is a continuation of application No. 16/649,476, granted, now 11,535,827, previously published as PCT/US2018/052315, filed on Sep. 22, 2018.
Claims priority of provisional application 62/562,127, filed on Sep. 22, 2017.
Prior Publication US 2023/0101122 A1, Mar. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/071 (2010.01); G01N 33/50 (2006.01)
CPC C12N 5/067 (2013.01) [G01N 33/5067 (2013.01); C12N 2502/1323 (2013.01); C12N 2502/28 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A product comprising plated hepatocytes on a surface, wherein at least 70% of the plated hepatocytes are in one or more hepatocyte clusters on feeder cells, wherein the feeder cells are endothelial cells and fibroblasts and are attached to the surface, wherein the plated hepatocytes are obtained from one human donor, one non-human donor, multiple human donors, or multiple non-human donors;
the endothelial cells are obtained from one human donor, one non-human donor, multiple human donors, or multiple non-human donors; and
the fibroblasts are obtained from one human donor, one non-human donor, multiple human donors, or multiple non-human donors;
and wherein the plated hepatocytes, the endothelial cells and the fibroblasts have a cell ratio from 3:1:1 to 24:1:1.