	US 12,215,344 B2
	Engineered multicellular ciliated organisms and kinematic self-replication thereof
Michael Levin, Beverly, MA (US); Douglas J. Blackiston, Medford, MA (US); Kelly A. McLaughlin, Medford, MA (US); Josh Bongard, Jericho, VT (US); and Sam Kriegman, Burlington, VT (US)
Assigned to Trustees of Tufts College, Medford, MA (US); and University of Vermont, Burlington, VT (US)
Filed by Trustees of Tufts College, Medford, MA (US); and University of Vermont, Burlington, VT (US)
Filed on Jan. 12, 2022, as Appl. No. 17/647,847.
Claims priority of provisional application 63/261,258, filed on Sep. 15, 2021.
Claims priority of provisional application 63/136,564, filed on Jan. 12, 2021.
Prior Publication US 2022/0220437 A1, Jul. 14, 2022
Int. Cl. C12N 5/07 (2010.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01)

CPC C12N 5/06 (2013.01) [C12M 21/08 (2013.01); C12M 23/16 (2013.01)]

17 Claims

1. An engineered multicellular organism comprising an aggregate of ciliated Xenopus laevis (X. laevis) cells derived from fertilized X. laevis embryonic cells, wherein the cilia of the ciliated cells move the organism at a rate of at least 15 microns/second in an aqueous environment.