US 12,215,339 B2
Lentivirus packaging system comprising a synthetic positive feedback loop
Toru Matsuura, Cincinnati, OH (US); Christian I. Hong, Cincinnati, OH (US); and Kaoru Matsuura, Cincinnati, OH (US)
Assigned to University of Cincinnati, Cincinnati, OH (US)
Filed by UNIVERSITY OF CINCINNATI, Cincinnati, OH (US)
Filed on Nov. 22, 2021, as Appl. No. 17/532,075.
Application 17/532,075 is a division of application No. 16/069,574, granted, now 11,208,669, previously published as PCT/US2017/013092, filed on Jan. 12, 2017.
Claims priority of provisional application 62/277,602, filed on Jan. 12, 2016.
Prior Publication US 2022/0154214 A1, May 19, 2022
Int. Cl. C12N 15/86 (2006.01); A61K 48/00 (2006.01); C07K 14/005 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 48/005 (2013.01); C07K 14/005 (2013.01); C12N 2740/15042 (2013.01); C12N 2740/15052 (2013.01); C12N 2740/16022 (2013.01); C12N 2740/16043 (2013.01); C12N 2740/16052 (2013.01); C12N 2830/003 (2013.01)] 2 Claims
 
1. A lentivirus packaging system comprising:
(a) a helper plasmid comprising tetracycline transactivator (tTA) under the control of tetracycline responsive element (TRE) (TRE-tTA);
(b) a CMV promotor-controlled tTA (CMV-tTA) helper plasmid;
(c) a CMV-VSV-G envelope plasmid; and
(d) at least one plasmid comprising a TRE promotor controlling a subset of HIV-1 genes, selected from the group consisting of:
a TRE-ΔR8.2-Δvpr plasmid, and
a TRE-ΔR8.2-Δvpr, rev, nef plasmid and a TRE-rev plasmid.