Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
Jay D. Keasling, Berkeley, CA (US); Leo D'Espaux, San Francisco, CA (US); Jeff Wong, Berkeley, CA (US); Xiaozhou Luo, Berkeley, CA (US); Michael Reiter, Berkeley, CA (US); Charles Denby, Berkeley, CA (US); and Anna Lechner, Berkeley, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Nov. 14, 2022, as Appl. No. 18/054,917.
Application 16/791,991 is a division of application No. 16/408,492, filed on May 10, 2019, granted, now 10,563,211, issued on Feb. 18, 2020.
Application 18/054,917 is a continuation of application No. 17/206,126, filed on Mar. 19, 2021, granted, now 11,542,512.
Application 17/206,126 is a continuation of application No. 16/791,991, filed on Feb. 14, 2020, granted, now 10,975,379, issued on Apr. 13, 2021.
Application 16/408,492 is a continuation of application No. PCT/US2018/029668, filed on Apr. 27, 2018.
Claims priority of provisional application 62/569,532, filed on Oct. 7, 2017.
Claims priority of provisional application 62/491,114, filed on Apr. 27, 2017.
Prior Publication US 2023/0340506 A1, Oct. 26, 2023
1. A genetically modified host cell for producing a cannabinoid or a cannabinoid derivative, the cell comprising one or more heterologous nucleic acids encoding a geranyl pyrophosphate:olivetolic acid geranyltransferase, wherein the geranyltransferase catalyzes in the cell production of cannabigerolic acid from geranyl pyrophosphate and olivetolic acid in an amount at least ten times higher than CsPT1 comprising the amino acid sequence of SEQ ID NO:82, wherein the geranyltransferase is Cannabis sativa CsPT4 comprising the amino acid sequence of SEQ ID NO:110 or a truncated version thereof.