US 12,215,324 B2
Monocyte and macrophage binding aptamers and their application
Suzie Hwang Pun, Seattle, WA (US); Meilyn Sylvestre, Seattle, WA (US); Nataly Kacherovsky, Seattle, WA (US); Emmeline Cheng, Seattle, WA (US); Ian Cardle, Seattle, WA (US); and Chris Saxby, Seattle, WA (US)
Assigned to University of Washington, Seattle, WA (US); and Seattle Children's Hospital, Seattle, WA (US)
Filed by University of Washington, Seattle, WA (US); and Seattle Children's Hospital, Seattle, WA (US)
Filed on Jun. 24, 2022, as Appl. No. 17/849,513.
Claims priority of provisional application 63/214,468, filed on Jun. 24, 2021.
Prior Publication US 2023/0017777 A1, Jan. 19, 2023
Int. Cl. C12N 15/115 (2010.01); A61K 39/00 (2006.01); C12N 5/0786 (2010.01); C12N 15/10 (2006.01)
CPC C12N 15/115 (2013.01) [A61K 39/4614 (2023.05); A61K 39/4622 (2023.05); A61K 39/4644 (2023.05); C12N 5/0645 (2013.01); C12N 15/1048 (2013.01); C12N 15/1065 (2013.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); C12N 2310/16 (2013.01)] 20 Claims
 
1. A composition comprising an aptamer that specifically binds to a monocyte and/or a macrophage, wherein the aptamer comprises a sequence having at least 75% sequence identity to SEQ ID NO:1; and wherein the aptamer can further comprise a number (N) of nucleotides (nt) at each end wherein each nucleotide is selected independently, and wherein each N comprises from 3 nt to 30 nt, from 3 nt to 20 nt, or from 3 nt to 10 nt;
wherein any nucleotide change in the sequence of the aptamer relative to SEQ ID NO:1 occurs within one or more stem regions of SEQ ID NO:1 and co-occurs with one or more compensatory nucleotide changes within the one or more stem regions of SEQ ID NO:1 to maintain double-strandedness of the one or more stem regions of SEQ ID NO:1.