	US 12,215,323 B2
	Exogenous control of mammalian gene expression through aptamer-mediated modulation of polyadenylation
Laising Yen, Pearland, TX (US); Liming Luo, Pearland, TX (US); and Pei-Wen Chao, Sugar Land, TX (US)
Assigned to Baylor College of Medicine, Houston, TX (US)
Filed by Baylor College of Medicine, Houston, TX (US)
Filed on Jan. 8, 2021, as Appl. No. 17/144,456.
Application 17/144,456 is a continuation of application No. 15/775,804, abandoned, previously published as PCT/US2016/061665, filed on Nov. 11, 2016.
Claims priority of provisional application 62/254,435, filed on Nov. 12, 2015.
Prior Publication US 2021/0310003 A1, Oct. 7, 2021
Int. Cl. C07H 21/04 (2006.01); C12N 15/115 (2010.01); C12N 15/67 (2006.01); A61K 48/00 (2006.01)

CPC C12N 15/115 (2013.01) [C12N 15/67 (2013.01); A61K 48/00 (2013.01)]

33 Claims

1. A system for modulating gene expression, comprising a polyA aptamer polynucleotide that comprises in a 5′ to 3′ direction:

a) at least one ligand-binding aptamer comprising at least one polyA cleavage signal therein; and

b) an expressible polynucleotide that is a reporter gene,

wherein:

(i) the at least one ligand-binding aptamer resides within the 5′ untranslated region (UTR) of the expressible polynucleotide;

(ii) the polyA aptamer polynucleotide comprises at least one U/UG rich region, at least one G rich region, or both of at least one U/UG rich region and at least one G rich region; and

(iii) in a 5′ to 3′ direction, the at least one poly A cleavage signal resides upstream of the at least one U/UG rich region and/or the at least one G rich region.