	US 12,215,319 B2
	Methods and compositions for targeted genomic insertion
Zhongying Chen, Research Triangle Park, NC (US); Mary-Dell Chilton, Research Triangle Park, NC (US); Shujie Dong, Research Triangle Park, NC (US); Qiudeng Que, Research Triangle Park, NC (US); and Heng Zhong, Research Triangle Park, NC (US)
Assigned to Syngenta Crop Protection AG, Basel (CH)
Appl. No. 16/634,215
Filed by SYNGENTA PARTICIPATIONS AG, Basel (CH)
PCT Filed Jul. 26, 2018, PCT No. PCT/US2018/043855 § 371(c)(1), (2) Date Jan. 27, 2020, PCT Pub. No. WO2019/027789, PCT Pub. Date Feb. 7, 2019.
Claims priority of provisional application 62/541,219, filed on Aug. 4, 2017.
Prior Publication US 2021/0087557 A1, Mar. 25, 2021
Int. Cl. C12N 15/113 (2010.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2018.01)

CPC C12N 15/113 (2013.01) [C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 2310/20 (2017.05)]

9 Claims

1. A chimeric RNA for targeted genomic modification of a cell, wherein the chimeric RNA comprises:

a) a guide RNA comprising a polynucleotide targeting guide sequence of at least 8 contiguous nucleotides and at least 80% identical to a target nucleic acid sequence of at least 8 contiguous nucleotides within a target nucleic acid molecule; and

b) a bait RNA comprising at least 8 contiguous nucleic acids, wherein the nucleic acid sequence of the bait RNA is at least 70% complementary to at least 8 contiguous nucleic acids of a donor DNA molecule, wherein the donor DNA molecule is intended for integration into the genome of a cell, wherein a tracrRNA is operably linked to said bait RNA sequence.