US 12,215,314 B2
Integrative DNA and RNA library preparations and uses thereof
Yexun Wang, Ellicott City, MD (US); Quan Peng, Clarksburg, MD (US); and Daniel Kim, Brunswick, MD (US)
Assigned to QIAGEN Sciences, LLC, Germantown, MD (US)
Appl. No. 17/041,724
Filed by Yexun Wang, Ellicott City, MD (US); Quan Peng, Clarksburg, MD (US); Daniel Kim, Brunswick, MD (US); and QIAGEN Sciences, LLC, Germantown, MD (US)
PCT Filed Mar. 26, 2019, PCT No. PCT/US2019/024107
§ 371(c)(1), (2) Date Sep. 25, 2020,
PCT Pub. No. WO2019/191122, PCT Pub. Date Oct. 3, 2019.
Claims priority of provisional application 62/648,174, filed on Mar. 26, 2018.
Prior Publication US 2021/0024920 A1, Jan. 28, 2021
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6806 (2018.01)
CPC C12N 15/1065 (2013.01) [C12Q 1/6806 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for preparing DNA and cDNA libraries in a same sample comprising DNA and RNA molecules, comprising:
ligating a DNA tag comprising a single-stranded portion to a 3′ end of a double-stranded DNA molecule in the same sample to form a tagged DNA, wherein the DNA tag comprises a unique molecular identifier (UMI) and a DNA identifier;
performing reverse transcription of a RNA molecule in the same sample in the presence of a RNA tag to form a tagged cDNA, wherein the RNA tag comprises a RNA identifier, a UMI, and a poly(T); and
generating DNA and cDNA libraries in the same sample.