CPC C12N 15/1065 (2013.01) [C12Q 1/6806 (2013.01)] | 16 Claims |
1. A method for preparing DNA and cDNA libraries in a same sample comprising DNA and RNA molecules, comprising:
ligating a DNA tag comprising a single-stranded portion to a 3′ end of a double-stranded DNA molecule in the same sample to form a tagged DNA, wherein the DNA tag comprises a unique molecular identifier (UMI) and a DNA identifier;
performing reverse transcription of a RNA molecule in the same sample in the presence of a RNA tag to form a tagged cDNA, wherein the RNA tag comprises a RNA identifier, a UMI, and a poly(T); and
generating DNA and cDNA libraries in the same sample.
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