US 12,215,312 B2
Modified organisms for improved flavor and aroma
Sean Raspet, Detroit, MI (US)
Filed by Sean Raspet, Detroit, MI (US)
Filed on May 5, 2022, as Appl. No. 17/737,035.
Application 17/737,035 is a division of application No. 16/383,086, filed on Apr. 12, 2019, granted, now 11,352,618.
Prior Publication US 2022/0267755 A1, Aug. 25, 2022
Int. Cl. C12N 15/10 (2006.01); C12N 9/00 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/102 (2013.01) [C12N 15/902 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] 10 Claims
 
1. A method of producing a better tasting organism comprising editing a genome of the organism to target production of at least one molecule selected from the group consisting of:
geosmin, 2-methylisoborneol, 2-pentylfuran, putrescine, cadaverine, spermine, spermidine, thermospermine, polyamines having a molecular weight below 350 Daltons, Beta-cyclocitral, 1-octen-3-ol, sulfides, thiomethyl esters, 2,4-decadienal, 1,3-Octadiene, Octatrienes, 4-Methylthio-1,2-dithiolane, 5-Methylthio-1,2,3-trithiane, Dimethyldisulfide, Dimethyltrisulfide, Methyl ethanethiolate, Methyl propanethiolate, Methyl 2-methyipropanethiolate, Methyl 3-methylbutanethiolate, Pentanal, Heptanal, Dimethyltetrasulfide, 1,2,4-Trithiolane, Oct-I-en-3-O-1, Octan-I-ol, Oct-2-en-1-ol, 1-octen-3-one, Octan-3-one, Benzene, Naphthalene, Hexanal, Pentan-3-one, Styrene, Octanal, Decanal, Nonanal, Undecanal, histamine, Thioesters, Uric acids, Uronic acids, Purines, Pinene, Limonene, Borneol, Fenchol, Indole, Skatole, polyunsaturated fatty acids, and free fatty acids,
wherein the editing of the genome of the organism occurs via a CRISPR system, or TALENs or zinc fingers,
wherein the organism is algae,
wherein the editing of the genome of the organism removes unwanted traits from the organism,
wherein the unwanted traits comprise at least one of aroma and taste,
wherein a targeted gene related to the production of the molecule is knocked-out, made non-functional, or is from the genome of the organism, or in the alternative,
wherein a targeted gene related to the production of the molecule is upregulated or downregulated as a result of the editing.