US 12,215,310 B2
Means and methods to modulate probiotic potency of the yeast Saccharomyces boulardii
Johan Thevelein, Blanden (BE); Benjamin Offei, Leuven (BE); Maria Remedios Foulquié Moreno, Brussels (BE); and Paul Vandecruys, Heusden-Zolder (BE)
Assigned to VIB VZW, Ghent (BE); and Katholieke Universiteit Leuven, Leuven (BE)
Appl. No. 16/646,936
Filed by VIB VZW, Ghent (BE); and KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D, Leuven (BE)
PCT Filed Sep. 14, 2018, PCT No. PCT/EP2018/074953
§ 371(c)(1), (2) Date Mar. 12, 2020,
PCT Pub. No. WO2019/053218, PCT Pub. Date Mar. 21, 2019.
Claims priority of application No. 17191252 (EP), filed on Sep. 15, 2017.
Prior Publication US 2020/0270568 A1, Aug. 27, 2020
Int. Cl. C12N 1/18 (2006.01); A23L 33/14 (2016.01); A61K 36/064 (2006.01); C07K 14/395 (2006.01); C12P 7/54 (2006.01)
CPC C12N 1/18 (2013.01) [A23L 33/14 (2016.08); A61K 36/064 (2013.01); C07K 14/395 (2013.01); C12P 7/54 (2013.01); A23V 2002/00 (2013.01)] 7 Claims
 
1. A Saccharomyces boulardii strain comprising a homozygous or hemizygous mutant WHI2 allele,
wherein the mutant allele completely abolishes Whi2 function,
wherein the wild type allele of Whi2 is SEQ ID NO: 3,
wherein the mutant allele
has been disrupted or deleted by homologous recombination or disrupted with a nuclease, and
wherein the strain produces, at 37° C., a cell-free supernatant with a pH lower than 5.