US 12,215,122 B2
Nucleotide analogues
Ronald Graham, Carlsbad, CA (US); Olga Adelfinskaya, San Marcos, CA (US); Megha Cila, San Diego, CA (US); Rodrigo Rodriguez, San Diego, CA (US); Abrehet Abdu, San Diego, CA (US); and Eli N. Glezer, Del Mar, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Nov. 29, 2023, as Appl. No. 18/522,886.
Application 18/522,886 is a continuation of application No. 18/295,770, filed on Apr. 4, 2023, granted, now 11,878,993.
Application 18/295,770 is a continuation of application No. 18/178,802, filed on Mar. 6, 2023, granted, now 11,958,877.
Application 18/178,802 is a continuation of application No. 17/287,255, granted, now 12,145,960, previously published as PCT/US2019/057842, filed on Oct. 24, 2019.
Claims priority of provisional application 62/841,146, filed on Apr. 30, 2019.
Claims priority of provisional application 62/789,877, filed on Jan. 8, 2019.
Claims priority of provisional application 62/750,552, filed on Oct. 25, 2018.
Prior Publication US 2024/0174709 A1, May 30, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C07H 19/10 (2006.01); C07H 19/14 (2006.01); C07H 19/20 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01)
CPC C07H 19/14 (2013.01) [C07H 19/10 (2013.01); C07H 19/20 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01)] 19 Claims
 
1. A method of sequencing a nucleic acid molecule comprising:
(a) incorporating in series a plurality of nucleotides into a primer hybridized to the nucleic acid molecule to create an extension strand, wherein each nucleotide comprises:
(i) a cleavable moiety covalently bound to the 3′ oxygen of the nucleotide, said cleavable moiety having the formula -L1P-S—S—R6P (I); wherein, L1P is a substituted methylene, wherein L1P is substituted with a substituted or unsubstituted alkyl, or a substituted or unsubstituted heteroalkyl; R6P is unsubstituted C1-C4 alkyl; and
(ii) a detectable moiety attached to said nucleotide via a covalent linker, wherein said covalent linker comprises

OG Complex Work Unit Chemistry
 wherein, R102 is unsubstituted C1-C4 alkyl; and
(b) detecting the detectable label of the incorporated nucleotide to identify the incorporated nucleotide in the extension strand, thereby sequencing the nucleic acid molecule.