US 12,214,347 B2
Nanopore/nanowell electrode enabled exonuclease sequencing
Anna E. P. Schibel, Snoqualmie, WA (US); Eric N. Ervin, Holladay, UT (US); and Sean German, Salt Lake City, UT (US)
Assigned to ELECTRONIC BIOSCIENCES, INC., San Diego, CA (US)
Filed by ELECTRONIC BIOSCIENCES, INC., San Diego, CA (US)
Filed on Aug. 30, 2023, as Appl. No. 18/240,253.
Application 18/240,253 is a continuation of application No. 16/605,728, granted, now 11,752,497, previously published as PCT/US2018/028200, filed on Apr. 18, 2018.
Claims priority of provisional application 62/487,414, filed on Apr. 19, 2017.
Prior Publication US 2023/0405580 A1, Dec. 21, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6869 (2018.01); B01L 3/00 (2006.01); G01N 33/487 (2006.01)
CPC B01L 3/5027 (2013.01) [C12Q 1/6869 (2013.01); G01N 33/48721 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0858 (2013.01); B01L 2400/0418 (2013.01); B01L 2400/0421 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for determining a polymer sequence, comprising:
a) electrophoretically and/or electroosmotically driving a polymer from a bulk solution through a transmembrane channel disposed in a membrane into a confined volume of a chamber; whereby the polymer contacts an enzyme in the confined volume of the chamber, under conditions in which the enzyme cleaves monomeric units from the polymer;
b) electrophoretically and/or electroosmotically driving monomeric units in the order which they are sequentially cleaved from the polymer by the enzyme out of the confined volume of the chamber through the transmembrane channel; and
c) determining the identity of each of the monomeric units based on its current signature, translocation time, and/or associated current noise level modulation as the monomeric unit translocates through the transmembrane channel, thereby determining the polymer sequence.