	US 12,214,046 B2
	Protein-loaded PLGA nanospheres
Brock A Lindsey, Morgantown, WV (US); Justin E Markel, Pittsburgh, PA (US); Ryan A Lacinski, Bridgeville, PA (US); and Jabeen Noore, Kingsville, TX (US)
Assigned to West Virginia University Board of Governors on behalf of West Virginia University, Morgantown, WV (US)
Filed by West Virginia University Board of Governors on behalf of West Virginia University, Morgantown, WV (US)
Filed on Jun. 2, 2022, as Appl. No. 17/830,615.
Application 17/830,615 is a continuation of application No. PCT/US2020/063314, filed on Dec. 4, 2020.
Claims priority of provisional application 62/944,191, filed on Dec. 5, 2019.
Prior Publication US 2023/0070180 A1, Mar. 9, 2023
Int. Cl. A61K 47/62 (2017.01); A61K 9/51 (2006.01); A61K 31/43 (2006.01); A61K 31/7036 (2006.01); A61K 38/20 (2006.01)

CPC A61K 47/62 (2017.08) [A61K 9/5153 (2013.01); A61K 31/43 (2013.01); A61K 31/7036 (2013.01); A61K 38/208 (2013.01)]

6 Claims

1. A PLGA nanosphere prepared by

a) forming a first emulsion comprising i) PLGA comprising from 50% to 90% lactide, ii) a sorbitan fatty acid ester, iii) an organic solvent, and iv) a first aqueous phase comprising A) IL-12 and B) a species-specific whole serum, a species-specific engineered serum albumin, or a species-specific native serum albumin;

b) forming a second emulsion comprising i) the first emulsion and ii) a second aqueous phase comprising A) polyvinyl alcohol (PVA) and B) a polyoxyethylene sorbitan fatty acid ester; and

c) evaporating the organic solvent from the second emulsion, thereby forming the PLGA nanosphere.