US 12,214,031 B2
Mannheimia haemolytica vector
Robert E. Briggs, Boone, IA (US); and Fred M. Tatum, Nevada, IA (US)
Assigned to The United States of America, as represented by the Secretary of Agriculture, Washington, DC (US)
Filed by The United States of America, as represented by The Secretary of Agriculture, Washington, DC (US)
Filed on May 2, 2023, as Appl. No. 18/310,821.
Application 18/310,821 is a division of application No. 17/190,182, filed on Mar. 2, 2021, granted, now 11,679,150.
Claims priority of provisional application 62/984,700, filed on Mar. 3, 2020.
Prior Publication US 2023/0330204 A1, Oct. 19, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/102 (2006.01); A61K 39/04 (2006.01); A61P 31/04 (2006.01); A61K 39/00 (2006.01)
CPC A61K 39/102 (2013.01) [A61K 39/04 (2013.01); A61P 31/04 (2018.01); A61K 2039/5256 (2013.01)] 10 Claims
 
1. A cassette comprising a modified Mannheimia haemolytica lktCA gene cluster, wherein the modified M. haemolytica lktCA gene cluster comprises two polynucleotides encoding leukotoxin neutralizing epitopes, a first polynucleotide encoding the native leukotoxin neutralizing epitope, and a second polynucleotide encoding an additional leukotoxin neutralizing epitope inserted immediately downstream of the native leukotoxin A start codon.