US 12,214,022 B2
Sequence-specific antimicrobials by blocking DNA repair
David Bikard, Paris (FR); Lun Cui, Paris (FR); Xavier Duportet, Paris (FR); and Jesus Fernandez Rodriguez, Paris (FR)
Assigned to INSTITUT PASTEUR, Paris (FR); and ELIGO BIOSCIENCE, Paris (FR)
Filed by INSTITUT PASTEUR, Paris (FR); and ELIGO BIOSCIENCE, Paris (FR)
Filed on Jun. 3, 2022, as Appl. No. 17/831,507.
Application 17/831,507 is a continuation of application No. 16/671,978, filed on Nov. 1, 2019, granted, now 11,357,831, issued on Jun. 14, 2022.
Application 16/671,978 is a continuation of application No. 15/744,039, abandoned, previously published as PCT/EP2016/066702, filed on Jul. 13, 2016.
Claims priority of provisional application 62/191,572, filed on Jul. 13, 2015.
Prior Publication US 2022/0347271 A1, Nov. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 38/46 (2006.01); A61P 31/04 (2006.01); C12N 1/20 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/63 (2006.01); C12N 15/70 (2006.01)
CPC A61K 38/465 (2013.01) [A61P 31/04 (2018.01); C12N 1/20 (2013.01); C12N 9/22 (2013.01); C12N 15/10 (2013.01); C12N 15/1024 (2013.01); C12N 15/63 (2013.01); C12N 15/70 (2013.01); C12N 2310/20 (2017.05); C12N 2795/10122 (2013.01); Y02A 50/30 (2018.01)] 16 Claims
OG exemplary drawing
 
1. A method for killing a bacterium comprising contacting the bacterium with at least one recombinant phagemid(s) or plasmid(s);
wherein the recombinant phagemid(s) or plasmid(s) encodes an endonuclease that creates a double-stranded break (DSB) in the chromosomal or extrachromosomal DNA of the bacterium, and
an exogenous protein that inhibits DSB repair.