	US 12,213,860 B2
	Method for separating mammalian sperm, artificial insemination method, and in vitro fertilization method
Masayuki Shimada, Hiroshima (JP); Takashi Umehara, Hiroshima (JP); Masaaki Goto, Oita (JP); and Moeka Kugumiya, Oita (JP)
Assigned to Hiroshima University, Higashihiroshima (JP)
Appl. No. 16/627,660
Filed by Hiroshima University, Higashihiroshima (JP)
PCT Filed Jun. 26, 2018, PCT No. PCT/JP2018/024206 § 371(c)(1), (2) Date Dec. 30, 2019, PCT Pub. No. WO2019/004217, PCT Pub. Date Jan. 3, 2019.
Claims priority of application No. 2017-129768 (JP), filed on Jun. 30, 2017; and application No. 2018-120260 (JP), filed on Jun. 25, 2018.
Prior Publication US 2020/0129280 A1, Apr. 30, 2020
Int. Cl. A61D 19/02 (2006.01); C12N 5/076 (2010.01); G01N 1/40 (2006.01)

CPC A61D 19/02 (2013.01) [C12N 5/061 (2013.01); G01N 1/4077 (2013.01); C12N 2501/999 (2013.01)]

9 Claims

1. A method for separating mammalian sperm, comprising

incubating the sperm in a medium containing a TLR7 ligand; and

separating the sperm into a population of X chromosome-bearing sperm that is sunk and a population of Y chromosome-bearing sperm that is floating, wherein

the TLR7 ligand causes the population of the X chromosome-bearing sperm that is to show a decrease in sperm activity in response to the TLR7 ligand to sink, and

the population of the Y chromosome-bearing sperm that is less likely to show a decrease in sperm activity in response to the TLR7 ligand floats upward.