US 12,213,784 B2
Durable enzyme-based biosensor and process for drop deposition immobilization
Yunqing Du, Quincy, MA (US); and Ming L. Wang, Ipswich, MA (US)
Assigned to Northeastern University, Boston, MA (US)
Appl. No. 16/468,146
Filed by Northeastern University, Boston, MA (US)
PCT Filed Dec. 11, 2017, PCT No. PCT/US2017/065624
§ 371(c)(1), (2) Date Jun. 10, 2019,
PCT Pub. No. WO2018/107168, PCT Pub. Date Jun. 14, 2018.
Claims priority of provisional application 62/432,513, filed on Dec. 9, 2016.
Prior Publication US 2020/0405200 A1, Dec. 31, 2020
Int. Cl. A61B 5/1486 (2006.01); A61B 5/145 (2006.01); G01N 27/327 (2006.01)
CPC A61B 5/1486 (2013.01) [A61B 5/14532 (2013.01); G01N 27/3271 (2013.01); A61B 2562/0285 (2013.01)] 23 Claims
OG exemplary drawing
 
1. A sensor for determining a concentration of a biomarker in a liquid sample, the sensor comprising:
an insulating or semiconducting substrate;
at least one working electrode, a counter electrode, and a reference electrode, and
a sample placement area on a surface of the substrate for containing the liquid sample during a determination of the biomarker concentration in the liquid sample;
wherein each of the working electrode(s), counter electrode, and reference electrode comprises a conductive metal layer deposited on the substrate in the sample placement area;
wherein the working electrode is coated in the sample placement area with a plurality of sensor elements;
wherein the sensor elements are functionalized with a functionalization coating comprising a quaternary ammonium compound, a plurality of metal nanoparticles, a crosslinked enzyme that catalyzes an electron transfer reaction specific for the biomarker;
wherein the working electrode, counter electrode, and reference electrode are connected to an amperometry circuit;
wherein the sensor measures electron transfer into the working electrode;
wherein an output voltage of the amperometry circuit correlates with concentration of the biomarker in the liquid sample deposited in the sample placement area; and
wherein the sensor has been fabricated by a method comprising drop deposition of the functionalization coating from a single suspension comprising the quaternary ammonium compound, the metal nanoparticles, and the enzyme.