	US 12,213,501 B2
	Methods for modifying high intensity sweetener glycosides
Stephen Patrick O'Hara, York (GB); Oswaldo Hernandez, York (GB); and Sofia Kolida, York (GB)
Assigned to Optibiotix Limited, York (GB)
Appl. No. 17/044,870
Filed by Optibiotix Limited, York (GB)
PCT Filed Apr. 4, 2019, PCT No. PCT/GB2019/050993 § 371(c)(1), (2) Date Oct. 2, 2020, PCT Pub. No. WO2019/193356, PCT Pub. Date Oct. 10, 2019.
Claims priority of application No. 1805576 (GB), filed on Apr. 4, 2018.
Prior Publication US 2021/0100272 A1, Apr. 8, 2021
Int. Cl. A23L 27/30 (2016.01); A23L 27/00 (2016.01); A23L 33/125 (2016.01); C12N 9/10 (2006.01)

CPC A23L 27/36 (2016.08) [A23L 27/86 (2016.08); A23L 33/125 (2016.08); C12N 9/1074 (2013.01); C12Y 204/01019 (2013.01); A23V 2002/00 (2013.01)]

5 Claims

1. A method for modifying a high intensity sweetener glycoside so as to reduce one or more off flavors, the method comprising:

a) contacting the high intensity sweetener glycoside with a cyclodextrin glycosyltransferase (CGTase) in the presence of maltodextrin under conditions effective to result in:

(i) at least a partial increase of glycoside chains of the high intensity sweetener glycoside; and/or

(ii) at least a partial increase of the glycoside chains of a portion of the high intensity sweetener glycoside;

so as to form modified high intensity sweetener glycosides or mixture of partially modified high intensity sweetener glycosides,

wherein the high intensity sweetener glycoside is a Mogroside.