US 12,213,420 B2
Molecular markers for blackleg resistance gene RLM7 in Brassica napus, and methods of using the same
Syed Masood Rizvi, Brampton (CA); Muhammad Tahir, Saskatoon (CA); Shunxue Tang, Carmel, IN (US); Jianwei Zhao, Saskatoon (CA); Jinhua Xiao, Eastvale, CA (US); and Gregory Gingera, Saskatoon (CA)
Assigned to CORTEVA AGRISCIENCE, LLC, Indianapolis, IN (US)
Appl. No. 17/266,730
Filed by Dow AgroSciences LLC, Indianapolis, IN (US)
PCT Filed Aug. 13, 2019, PCT No. PCT/US2019/046344
§ 371(c)(1), (2) Date Feb. 8, 2021,
PCT Pub. No. WO2020/036954, PCT Pub. Date Feb. 20, 2020.
Claims priority of provisional application 62/718,045, filed on Aug. 13, 2018.
Prior Publication US 2021/0315177 A1, Oct. 14, 2021
Int. Cl. A01H 1/04 (2006.01); A01H 1/00 (2006.01); A01H 6/20 (2018.01); C12Q 1/6895 (2018.01)
CPC A01H 1/045 (2021.01) [A01H 1/125 (2021.01); A01H 6/202 (2018.05); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] 10 Claims
 
1. A method for marker assisted selection to produce a progeny Brassica napus plant or germplasm that comprises Rlm7 locus for blackleg resistance, the method comprising:
assaying isolated nucleic acids from a first Brassica napus plant or germplasm for genomic markers thereof;
detecting in the isolated material at least one marker linked to Rlm7 locus for blackleg resistance, wherein the at least one marker is a single nucleotide polymorphism (SNP) selected from the group consisting of DBSNP216588 shown in SEQ ID NO:11, DBSNP216601 shown in SEQ ID NO:12, DBSNP216602 shown in SEQ ID NO:13, DBSNP216603 shown in SEQ ID NO:14, DBSNP216604 shown in SEQ ID NO:15, DBSNP216600 shown in SEQ ID NO:16, DBSNP216606 shown in SEQ ID NO:17, DBSNP216607 shown in SEQ ID NO:18, and DBSNP216608 shown in SEQ ID NO: 19;
selecting the first Brassica napus plant or germplasm thereof based on the detection of the at least one marker linked to blackleg resistance in its isolated nucleic acids; and
crossing the selected first Brassica napus plant or germplasm with a second Brassica plant or germplasm to thereby produce progeny Brassica napus plant or germplasm comprising the at least one marker linked to blackleg resistance.