US 11,885,953 B2
Apparatus and method of providing parameter estimation
Austin Corbett, San Diego, CA (US); Bo Lu, San Diego, CA (US); Robert Langlois, San Diego, CA (US); Joseph Pinto, Solana Beach, CA (US); Yu Chen, Irvine, CA (US); Peter Newman, San Diego, CA (US); and Hongji Ren, San Diego, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Dec. 22, 2021, as Appl. No. 17/558,829.
Application 17/558,829 is a continuation of application No. 17/110,406, filed on Dec. 3, 2020, granted, now 11,243,390.
Claims priority of provisional application 62/944,692, filed on Dec. 6, 2019.
Prior Publication US 2022/0113532 A1, Apr. 14, 2022
Int. Cl. G06K 9/00 (2022.01); G02B 21/36 (2006.01); G06T 7/80 (2017.01); G02B 26/06 (2006.01); G06T 7/00 (2017.01); H04N 23/56 (2023.01); G02B 21/08 (2006.01)
CPC G02B 21/365 (2013.01) [G02B 26/06 (2013.01); G06T 7/0014 (2013.01); G06T 7/80 (2017.01); H04N 23/56 (2023.01); G02B 21/082 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30168 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method including:
receiving a plurality of image sets, each image set of the plurality of image sets including images captured using structured illumination microscopy (SIM) in an optical system;
isolating a first image set from the plurality of image sets;
measuring a full width at half maximum (FWHM) value corresponding to each image in the first image set;
performing parameter estimation based at least in part on the measured FWHM value corresponding to each image the first image set;
identifying in-focus parameters based at least in part on the performed parameter estimation corresponding to the first image set;
calculating a co-focus metric for the image sets; and
generating a report presenting parameter values corresponding to the optical system, based at least in part on the calculated co-focus metrics.