US 11,885,952 B2
Optics, device, and system for assaying and imaging
Stephen Y. Chou, Princeton, NJ (US); Wei Ding, East Windsor, NJ (US); Ji Qi, Hillsborough, NJ (US); Yuecheng Zhang, Yardley, PA (US); Wu Chou, Basking Ridge, NJ (US); Mingquan Wu, Princeton Junction, NJ (US); Xing Li, Metuchen, NJ (US); and Jun Tian, Belle Mead, NJ (US)
Assigned to Essenlix Corporation, Monmouth Junction, NJ (US)
Filed by Essenlix Corporation, Monmouth Junction, NJ (US)
Filed on Jul. 30, 2019, as Appl. No. 16/526,864.
Claims priority of provisional application 62/716,911, filed on Aug. 9, 2018.
Claims priority of provisional application 62/716,353, filed on Aug. 8, 2018.
Claims priority of provisional application 62/716,338, filed on Aug. 8, 2018.
Claims priority of provisional application 62/712,204, filed on Jul. 30, 2018.
Prior Publication US 2020/0033579 A1, Jan. 30, 2020
Int. Cl. G02B 21/34 (2006.01); G01N 33/543 (2006.01); G01N 33/50 (2006.01)
CPC G02B 21/34 (2013.01) [G01N 33/54386 (2013.01); G01N 33/5094 (2013.01); G01N 2496/05 (2013.01)] 32 Claims
OG exemplary drawing
 
1. A method of assaying an analyte in a sample comprising:
(i) having a sample holder with a sample contact area for contacting a sample containing or suspected of containing an analyte;
(ii) having a plurality of calibration structures in the sample contact area of the sample holder, wherein the calibration structures have definite and predetermined geometry, and each of the calibration structures has an average lateral dimension of 100 μm or less;
(iii) imaging a part of the sample contact area that has the calibration structures with an imager to obtain an image; and
(iv) using an algorithm that comprises (a) the image in (iii), (b) the calibration structures in the image, and (c) machine learning to (1) identify the analyte, or (2) determine the analyte concentration, or (3) both (1) and (2).